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The conditions of cell-free systems (see Figs. 6 and 7) are such as to exclude any difference of activity which does not arise from the ribosomes themselves. The source and amount of pH 5 enzyme (containing polymerization and termination factors, together with iRNAs and aminoacyl synthetases) is the same, prepared from ribosome-free mouse liver homogenate, and does not limit the rate of polymerization. Thus, maturing erythroid cells accumulate polyribosomes that are inactive because incapable of releasing runoff 80 S monomers under conditions of protein synthesis.
That for 3. 22 times that of S. 09 pg per haploid set. Neither von Ledebur-Villiger (1972) nor Brookbank and Cummins (1972) report microspectrophotometric values for embryonic cells with more than the 4c amount of DNA. The kinetic complexity of DNA from echinoid sperm shows no unusual features. Cot curves (Britten and Kohne, 1968) indicate that some of the DNA reanneals a t rates indicating that it contains segments of very low complexity and high levels of reiteration, characteristic of satellite DNA or of internal reverse repeats (Wilson and Thomas, 1974).
11. Organization and Transcripti A. Characteristics of the DN ...................... A. Consideration of Methods.. Larvae . . . . . . . . . . . . . . . . . . . IV. Summary Statement, , 39 40 40 42 45 46 49 49 50 52 60 63 65 69 80 84 1. Introduction In the intensive analysis that has been made of the timing and regulation of the expression of the genome during the development of the echinoderm, many methods and approaches have been used. While i t is possible that the broad events of development are derived from the expression of a single or a f e w genes of critical import in the same sense that the phenotypic expression of an inheritable trait is relatable to a gene locus, an alternative possibility is that the major steps of embryonic determination, differentiation, and morphogenesis are brought about by the concerted effects of many genes, acting coordinately, triggered by external events or internal clocks.
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